Left: light-sheet and confocal images show vagal sensory neurons innervating the mouse heart and forming stereotyped nerve terminals. Right: a mouse tilt-table assay shows posture-dependent blood pressure changes and reduced recovery in vagal PIEZO2 knockout mice

When we stand up, gravity shifts blood toward the lower body, reducing the amount of blood returning to the heart. The nervous system initiates rapid reflexes to counteract gravity-induced effects, and dysfunction of this neural compensation can lead to postural hypotension, a sustained decrease in blood pressure that causes dizziness or fainting. However, the specific neural pathways that detect posture-related changes in circulation have been difficult to disentangle. 

In this study published in Nature, the Liberles Lab identified a small population of vagal sensory neurons that innervate the heart and act as blood volume receptors. These neurons express the mechanosensitive ion channel PIEZO2 and form distinctive end-net endings in the heart. Using mouse tilt-table experiments, anatomical mapping, in vivo electrophysiology and calcium imaging, and targeted genetic gain/loss-of-function approaches, the team shows that this vagal pathway is required for normal compensation to upright posture. 

Notably, the identified vagal PIEZO2 neurons produce heartbeat-coupled signals that track central blood volume and are time-locked to phases of the cardiac cycle. Selective knockout of PIEZO2 eliminates this heartbeat-coupled activity, causes postural hypotension, and compromises cardiovascular stability during trauma-induced blood loss. The work also demonstrates that optogenetic stimulation of these vagal fibers can lower blood pressure, supporting a causal role in hemodynamic control. 

Together, these findings reveal an essential vagal reflex that defends the constancy of circulation during posture change and hemorrhage, expanding our understanding of heart-brain communication in cardiovascular homeostasis.

Read the full publication here.