Steven Gygi, Ph.D.
Steven Gygi, Ph.D.
Professor of Cell Biology (HMS)

Steven Gygi, Ph.D., received his Ph.D. from the University of Utah in Pharmacology and Toxicology performing small molecule mass spectrometry.  He went on to pursue postdoctoral work with Ruedi Aebersold at the University of Washington in 1996.  A revolution in biological mass spectrometry was occurring which allowed for the measurement of protein expression levels and a new field, Proteomics, was born.  In 2000, Dr. Gygi moved to Harvard Medical School and joined the Department of Cell Biology.  Currently, he is the faculty director of two MS core facilities (Taplin Biological MS Facility, and the Thermo Fisher Center for Multiplexed Proteomics—TCMP@HMS).

Research in the Gygi lab centers around developing and applying new technologies in the field of mass spectrometry-based proteomics.  These include the systematic and proteome-wide measurements of many protein properties including their expression levels, modification states, structure, localization, function, and interactions.  For example, the Gygi lab, together with the Harper lab at HMS, is creating a genome-scale map of the protein-protein interaction landscape in cells (termed BioPlex).  In addition, sample multiplexing techniques like Tandem Mass Tags (TMT) are being improved to allow up to 16 proteomics samples to be analyzed simultaneously using high resolution mass spectrometry.

Harvard Medical School

Dept. of Cell Biology, C-523B

240 Longwood Avenue

Boston, MA 02115

Lab telephone: 617-432-3155

Lab fax: 617-432-1144

Assessing interference in isobaric tag-based sample multiplexing using an 18-plex interference standard.
Authors: Authors: Navarrete-Perea J, Liu X, Rad R, Gygi JP, Gygi SP, Paulo JA.
View full abstract on Pubmed
Rixosomal RNA degradation contributes to silencing of Polycomb target genes.
Authors: Authors: Zhou H, Stein CB, Shafiq TA, Shipkovenska G, Kalocsay M, Paulo JA, Zhang J, Luo Z, Gygi SP, Adelman K, Moazed D.
View full abstract on Pubmed
Functional mapping of PHF6 complexes in chromatin remodeling, replication dynamics and DNA repair.
Authors: Authors: Alvarez S, da Silva Almeida A, Albero R, Biswas M, Barreto-Galvez A, Gunning TS, Shaikh A, Aparicio T, Wendorff AA, Piovan E, Van Vlierberghe P, Gygi S, Gautier J, Madireddy A, Ferrando AA.
View full abstract on Pubmed
Fe3+-NTA magnetic beads as an alternative to spin column-based phosphopeptide enrichment.
Authors: Authors: Liu X, Rossio V, Thakurta SG, Flora A, Foster L, Bomgarden RD, Gygi SP, Paulo JA.
J Proteomics
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Kinase domain autophosphorylation rewires the activity and substrate specificity of CK1 enzymes.
Authors: Authors: Cullati SN, Chaikuad A, Chen JS, Gebel J, Tesmer L, Zhubi R, Navarrete-Perea J, Guillen RX, Gygi SP, Hummer G, Dötsch V, Knapp S, Gould KL.
Mol Cell
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NAD+ depletion enhances reovirus-induced oncolysis in multiple myeloma.
Authors: Authors: Kennedy BE, Giacomantonio M, Murphy JP, Cutler S, Sadek M, Konda P, Paulo JA, Pathak GP, Renkens SHJ, Grieve S, Pol J, Gygi SP, Richardson C, Gaston D, Reiman A, Kroemer G, Elnenaei MO, Gujar SA.
Mol Ther Oncolytics
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Genome-wide transcript and protein analysis highlights the role of protein homeostasis in the aging mouse heart.
Authors: Authors: Gerdes Gyuricza I, Chick JM, Keele GR, Deighan AG, Munger SC, Korstanje RC, Gygi SP, Churchill GA.
Genome Res
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Reuterin in the healthy gut microbiome suppresses colorectal cancer growth through altering redox balance.
Authors: Authors: Bell HN, Rebernick RJ, Goyert J, Singhal R, Kuljanin M, Kerk SA, Huang W, Das NK, Andren A, Solanki S, Miller SL, Todd PK, Fearon ER, Lyssiotis CA, Gygi SP, Mancias JD, Shah YM.
Cancer Cell
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Enrichment of Tyrosine Phosphorylated Peptides for Quantitative Mass Spectrometry Analysis of RTK Signaling Dynamics.
Authors: Authors: Vemulapalli V, Blacklow SC, Gygi SP, Erickson A.
Bio Protoc
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Interrogating Kinase-Substrate Relationships with Proximity Labeling and Phosphorylation Enrichment.
Authors: Authors: Zhang T, Fassl A, Vaites LP, Fu S, Sicinski P, Paulo JA, Gygi SP.
J Proteome Res
View full abstract on Pubmed