Steven Gygi, Ph.D.

Steven Gygi, Ph.D.

Professor of Cell Biology (HMS)
C-523C

Steven Gygi, Ph.D., received his Ph.D. from the University of Utah in Pharmacology and Toxicology performing small molecule mass spectrometry.  He went on to pursue postdoctoral work with Ruedi Aebersold at the University of Washington in 1996.  A revolution in biological mass spectrometry was occurring which allowed for the measurement of protein expression levels and a new field, Proteomics, was born.  In 2000, Dr. Gygi moved to Harvard Medical School and joined the Department of Cell Biology.  Currently, he is the faculty director of two MS core facilities (Taplin Biological MS Facility, and the Thermo Fisher Center for Multiplexed Proteomics—TCMP@HMS).

Research in the Gygi lab centers around developing and applying new technologies in the field of mass spectrometry-based proteomics.  These include the systematic and proteome-wide measurements of many protein properties including their expression levels, modification states, structure, localization, function, and interactions.  For example, the Gygi lab, together with the Harper lab at HMS, is creating a genome-scale map of the protein-protein interaction landscape in cells (termed BioPlex).  In addition, sample multiplexing techniques like Tandem Mass Tags (TMT) are being improved to allow up to 16 proteomics samples to be analyzed simultaneously using high resolution mass spectrometry.

Harvard Medical School

Dept. of Cell Biology, C-523B

240 Longwood Avenue

Boston, MA 02115

Expanding the landscape of aging via orbitrap astral mass spectrometry and tandem mass tag integration.
Authors: Authors: Keele GR, Dou Y, Kodikara SP, Jeffery ED, Bai DL, Hultenius E, Gao Z, Paulo JA, Gygi SP, Tian X, Zhang T.
Nat Commun
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ALS-related p97 R155H mutation disrupts lysophagy in iPSC-derived motor neurons.
Authors: Authors: Klickstein JA, Johnson MA, Antonoudiou P, Maguire J, Paulo JA, Gygi SP, Weihl C, Raman M.
Stem Cell Reports
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A dual-purification system to isolate mitochondrial subpopulations.
Authors: Authors: Cunningham CN, Van Vranken JG, Larios J, Heyden K, Gygi SP, Rutter J.
J Cell Sci
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The network response to Egf is tissue-specific.
Authors: Authors: Awasthi BW, Paulo JA, Burkhart DL, Smith IR, Collins RL, Harper JW, Gygi SP, Haigis KM.
iScience
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SCFFbw7 Modulates the NF?B Signaling Pathway by Targeting NF?B2 for Ubiquitination and Destruction.
Authors: Authors: Fukushima H, Matsumoto A, Inuzuka H, Zhai B, Lau AW, Wan L, Gao D, Shaik S, Yuan M, Gygi SP, Jimi E, Asara JM, Nakayama K, Nakayama KI, Wei W.
Cell Rep
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Multimodal cell maps as a foundation for structural and functional genomics.
Authors: Authors: Schaffer LV, Hu M, Qian G, Moon KM, Pal A, Soni N, Latham AP, Pontano Vaites L, Tsai D, Mattson NM, Licon K, Bachelder R, Cesnik A, Gaur I, Le T, Leineweber W, Palar A, Pulido E, Qin Y, Zhao X, Churas C, Lenkiewicz J, Chen J, Ono K, Pratt D, Zage P, Echeverria I, Sali A, Harper JW, Gygi SP, Foster LJ, Huttlin EL, Lundberg E, Ideker T.
Nature
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TMT-Based Multiplexed (Chemo)Proteomics on the Orbitrap Astral Mass Spectrometer.
Authors: Authors: He Y, Yang K, Li S, Zeller M, McAlister GC, Stewart HI, Hock C, Damoc E, Zabrouskov V, Gygi SP, Paulo JA, Yu Q.
Mol Cell Proteomics
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Deazaguanylation is a nucleobase-protein conjugation required for type IV CBASS immunity.
Authors: Authors: Wassarman DR, Pfaff P, Paulo JA, Gygi SP, Shokat KM, Kranzusch PJ.
bioRxiv
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Ergothioneine controls mitochondrial function and exercise performance via direct activation of MPST.
Authors: Authors: Sprenger HG, Mittenbühler MJ, Sun Y, Van Vranken JG, Schindler S, Jayaraj A, Khetarpal SA, Smythers AL, Vargas-Castillo A, Puszynska AM, Spinelli JB, Armani A, Kunchok T, Ryback B, Seo HS, Song K, Sebastian L, O'Young C, Braithwaite C, Dhe-Paganon S, Burger N, Mills EL, Gygi SP, Paulo JA, Arthanari H, Chouchani ET, Sabatini DM, Spiegelman BM.
Cell Metab
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Proteostasis and lysosomal repair deficits in transdifferentiated neurons of Alzheimer's disease.
Authors: Authors: Chou CC, Vest R, Prado MA, Wilson-Grady J, Paulo JA, Shibuya Y, Moran-Losada P, Lee TT, Luo J, Gygi SP, Kelly JW, Finley D, Wernig M, Wyss-Coray T, Frydman J.
Nat Cell Biol
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