Steven Gygi, Ph.D.

Steven Gygi, Ph.D.

Professor of Cell Biology (HMS)

Steven Gygi, Ph.D., received his Ph.D. from the University of Utah in Pharmacology and Toxicology performing small molecule mass spectrometry.  He went on to pursue postdoctoral work with Ruedi Aebersold at the University of Washington in 1996.  A revolution in biological mass spectrometry was occurring which allowed for the measurement of protein expression levels and a new field, Proteomics, was born.  In 2000, Dr. Gygi moved to Harvard Medical School and joined the Department of Cell Biology.  Currently, he is the faculty director of two MS core facilities (Taplin Biological MS Facility, and the Thermo Fisher Center for Multiplexed Proteomics—TCMP@HMS).

Research in the Gygi lab centers around developing and applying new technologies in the field of mass spectrometry-based proteomics.  These include the systematic and proteome-wide measurements of many protein properties including their expression levels, modification states, structure, localization, function, and interactions.  For example, the Gygi lab, together with the Harper lab at HMS, is creating a genome-scale map of the protein-protein interaction landscape in cells (termed BioPlex).  In addition, sample multiplexing techniques like Tandem Mass Tags (TMT) are being improved to allow up to 16 proteomics samples to be analyzed simultaneously using high resolution mass spectrometry.

Harvard Medical School

Dept. of Cell Biology, C-523B

240 Longwood Avenue

Boston, MA 02115

Lab telephone: 617-432-3155

Lab fax: 617-432-1144

Cell growth and nutrient availability control the mitotic exit signaling network in budding yeast.
Authors: Authors: Talavera RA, Prichard BE, Sommer RA, Leitao RM, Sarabia CJ, Hazir S, Paulo JA, Gygi SP, Kellogg DR.
J Cell Biol
View full abstract on Pubmed
Discovery of a DCAF11-dependent cyanoacrylamide-containing covalent degrader of BET-proteins.
Authors: Authors: Tin G, Cigler M, Hinterndorfer M, Dong KD, Imrichova H, Gygi SP, Winter GE.
Bioorg Med Chem Lett
View full abstract on Pubmed
Specificity profiling of deubiquitylases against endogenously generated ubiquitin-protein conjugates.
Authors: Authors: Rossio V, Paulo JA, Liu X, Gygi SP, King RW.
Cell Chem Biol
View full abstract on Pubmed
RNA quality control factors nucleate Clr4/SUV39H and trigger constitutive heterochromatin assembly.
Authors: Authors: Khanduja JS, Joh RI, Perez MM, Paulo JA, Palmieri CM, Zhang J, Gulka AOD, Haas W, Gygi SP, Motamedi M.
Cell
View full abstract on Pubmed
Genomic context- and H2AK119 ubiquitination-dependent inheritance of human Polycomb silencing.
Authors: Authors: Shafiq TA, Yu J, Feng W, Zhang Y, Zhou H, Paulo JA, Gygi SP, Moazed D.
Sci Adv
View full abstract on Pubmed
Ergothioneine boosts mitochondrial respiration and exercise performance via direct activation of MPST.
Authors: Authors: Sprenger HG, Mittenbühler MJ, Sun Y, Van Vranken JG, Schindler S, Jayaraj A, Khetarpal SA, Vargas-Castillo A, Puszynska AM, Spinelli JB, Armani A, Kunchok T, Ryback B, Seo HS, Song K, Sebastian L, O'Young C, Braithwaite C, Dhe-Paganon S, Burger N, Mills EL, Gygi SP, Arthanari H, Chouchani ET, Sabatini DM, Spiegelman BM.
bioRxiv
View full abstract on Pubmed
Inserting Pre-analytical Chromatographic Priming Runs Significantly Improves Targeted Pathway Proteomics with Sample Multiplexing.
Authors: Authors: Shuken SR, Yu Q, Gygi SP.
J Proteome Res
View full abstract on Pubmed
Bidirectional substrate shuttling between the 26S proteasome and the Cdc48 ATPase promotes protein degradation.
Authors: Authors: Li H, Ji Z, Paulo JA, Gygi SP, Rapoport TA.
Mol Cell
View full abstract on Pubmed
Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenation.
Authors: Authors: Mitchell W, Goeminne LJE, Tyshkovskiy A, Zhang S, Chen JY, Paulo JA, Pierce KA, Choy AH, Clish CB, Gygi SP, Gladyshev VN.
Elife
View full abstract on Pubmed
Inserting Pre-Analytical Chromatographic Priming Runs Significantly Improves Targeted Pathway Proteomics With Sample Multiplexing.
Authors: Authors: Shuken SR, Yu Q, Gygi SP.
bioRxiv
View full abstract on Pubmed