Steven Gygi
Steven Gygi, Ph.D.
Professor of Cell Biology (HMS)

Steven Gygi, Ph.D., received his Ph.D. from the University of Utah in Pharmacology and Toxicology performing small molecule mass spectrometry.  He went on to pursue postdoctoral work with Ruedi Aebersold at the University of Washington in 1996.  A revolution in biological mass spectrometry was occurring which allowed for the measurement of protein expression levels and a new field, Proteomics, was born.  In 2000, Dr. Gygi moved to Harvard Medical School and joined the Department of Cell Biology.  Currently, he is the faculty director of two MS core facilities (Taplin Biological MS Facility, and the Thermo Fisher Center for Multiplexed Proteomics—TCMP@HMS).

Research in the Gygi lab centers around developing and applying new technologies in the field of mass spectrometry-based proteomics.  These include the systematic and proteome-wide measurements of many protein properties including their expression levels, modification states, structure, localization, function, and interactions.  For example, the Gygi lab, together with the Harper lab at HMS, is creating a genome-scale map of the protein-protein interaction landscape in cells (termed BioPlex).  In addition, sample multiplexing techniques like Tandem Mass Tags (TMT) are being improved to allow up to 16 proteomics samples to be analyzed simultaneously using high resolution mass spectrometry.

Harvard Medical School

Dept. of Cell Biology, C-523B

240 Longwood Avenue

Boston, MA 02115

Lab telephone: 617-432-3155

Lab fax: 617-432-1144

Strain-Specific Peptide (SSP) Interference Reference Sample: A Genetically Encoded Quality Control for Isobaric Tagging Strategies.
Authors: Authors: Zhang T, Keele GR, Churchill GA, Gygi SP, Paulo JA.
Anal Chem
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DPP9 sequesters the C terminus of NLRP1 to repress inflammasome activation.
Authors: Authors: Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H.
Nature
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Peroxisomal-derived ether phospholipids link nucleotides to respirasome assembly.
Authors: Authors: Bennett CF, O'Malley KE, Perry EA, Balsa E, Latorre-Muro P, Riley CL, Luo C, Jedrychowski M, Gygi SP, Puigserver P.
Nat Chem Biol
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Super Heavy TMTpro Labeling Reagent: An Alternative and Higher-Charge-State-Amenable Stable-Isotope-Labeled TMTpro Variant.
Authors: Authors: Ordureau A, Yu Q, Bomgarden RD, Rogers JC, Harper JW, Gygi SP, Paulo JA.
J Proteome Res
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Proteomic and transcriptomic profiling reveal different aspects of aging in the kidney.
Authors: Authors: Takemon Y, Chick JM, Gerdes Gyuricza I, Skelly DA, Devuyst O, Gygi SP, Churchill GA, Korstanje R.
Elife
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Dual DNA and protein tagging of open chromatin unveils dynamics of epigenomic landscapes in leukemia.
Authors: Authors: Lee JD, Paulo JA, Posey RR, Mugoni V, Kong NR, Cheloni G, Lee YR, Slack FJ, Tenen DG, Clohessy JG, Gygi SP, Pandolfi PP.
Nat Methods
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Categorization of Phosphorylation Site Behavior during the Diauxic Shift in Saccharomyces cerevisiae.
Authors: Authors: Gassaway BM, Paulo JA, Gygi SP.
J Proteome Res
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A cold-stress-inducible PERK/OGT axis controls TOM70-assisted mitochondrial protein import and cristae formation.
Authors: Authors: Latorre-Muro P, O'Malley KE, Bennett CF, Perry EA, Balsa E, Tavares CDJ, Jedrychowski M, Gygi SP, Puigserver P.
Cell Metab
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Rapid toxin sequestration modifies poison frog physiology.
Authors: Authors: O'Connell LA, O'Connell JD, Paulo JA, Trauger SA, Gygi SP, Murray AW.
J Exp Biol
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Enrichment of Neurodegenerative Microglia Signature in Brain-Derived Extracellular Vesicles Isolated from Alzheimer's Disease Mouse Models.
Authors: Authors: Muraoka S, Jedrychowski MP, Iwahara N, Abdullah M, Onos KD, Keezer KJ, Hu J, Ikezu S, Howell GR, Gygi SP, Ikezu T.
J Proteome Res
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