Steven Gygi, Ph.D.

Steven Gygi, Ph.D.

Professor of Cell Biology (HMS)

Steven Gygi, Ph.D., received his Ph.D. from the University of Utah in Pharmacology and Toxicology performing small molecule mass spectrometry.  He went on to pursue postdoctoral work with Ruedi Aebersold at the University of Washington in 1996.  A revolution in biological mass spectrometry was occurring which allowed for the measurement of protein expression levels and a new field, Proteomics, was born.  In 2000, Dr. Gygi moved to Harvard Medical School and joined the Department of Cell Biology.  Currently, he is the faculty director of two MS core facilities (Taplin Biological MS Facility, and the Thermo Fisher Center for Multiplexed Proteomics—TCMP@HMS).

Research in the Gygi lab centers around developing and applying new technologies in the field of mass spectrometry-based proteomics.  These include the systematic and proteome-wide measurements of many protein properties including their expression levels, modification states, structure, localization, function, and interactions.  For example, the Gygi lab, together with the Harper lab at HMS, is creating a genome-scale map of the protein-protein interaction landscape in cells (termed BioPlex).  In addition, sample multiplexing techniques like Tandem Mass Tags (TMT) are being improved to allow up to 16 proteomics samples to be analyzed simultaneously using high resolution mass spectrometry.

Harvard Medical School

Dept. of Cell Biology, C-523B

240 Longwood Avenue

Boston, MA 02115

Lab telephone: 617-432-3155

Lab fax: 617-432-1144

Substrate identification and specificity profiling of deubiquitylases against endogenously-generated ubiquitin-protein conjugates.
Authors: Authors: Rossio V, Paulo JA, Liu X, Gygi SP, King RW.
bioRxiv
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Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input.
Authors: Authors: Yang K, Whitehouse RL, Dawson SL, Zhang L, Martin JG, Johnson DS, Paulo JA, Gygi SP, Yu Q.
Cell Chem Biol
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Quantitative proteomics defines mechanisms of antiviral defence and cell death during modified vaccinia Ankara infection.
Authors: Authors: Albarnaz JD, Kite J, Oliveira M, Li H, Di Y, Christensen MH, Paulo JA, Antrobus R, Gygi SP, Schmidt FI, Huttlin EL, Smith GL, Weekes MP.
Nat Commun
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Structural insights into cytokine cleavage by inflammatory caspase-4.
Authors: Authors: Devant P, Dong Y, Mintseris J, Ma W, Gygi SP, Wu H, Kagan JC.
Nature
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Ran-Binding Protein 3 Phosphorylation Links the Ras and PI3-Kinase Pathways to Nucleocytoplasmic Transport.
Authors: Authors: Yoon SO, Shin S, Liu Y, Ballif BA, Woo MS, Gygi SP, Blenis J.
Mol Cell
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Enhancing Proteome Coverage by Using Strong Anion-Exchange in Tandem with Basic-pH Reversed-Phase Chromatography for Sample Multiplexing-Based Proteomics.
Authors: Authors: Zhang T, Liu X, Rossio V, Dawson SL, Gygi SP, Paulo JA.
J Proteome Res
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High-Level Secretion of Pregnancy Zone Protein Is a Novel Biomarker of DNA Damage-Induced Senescence and Promotes Spontaneous Senescence.
Authors: Authors: Hu Z, Zhang M, Fan J, Hu J, Lin G, Piao S, Liu P, Liu J, Fu S, Sun W, Gygi SP, Zhang J, Zhou C.
J Proteome Res
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Targeting MCL-1 triggers DNA damage and an anti-proliferative response independent from apoptosis induction.
Authors: Authors: Adhikary U, Paulo JA, Godes M, Roychoudhury S, Prew MS, Ben-Nun Y, Yu EW, Budhraja A, Opferman JT, Chowdhury D, Gygi SP, Walensky LD.
Cell Rep
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A disordered region controls cBAF activity via condensation and partner recruitment.
Authors: Authors: Patil A, Strom AR, Paulo JA, Collings CK, Ruff KM, Shinn MK, Sankar A, Cervantes KS, Wauer T, St Laurent JD, Xu G, Becker LA, Gygi SP, Pappu RV, Brangwynne CP, Kadoch C.
Cell
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The anaphase-promoting complex controls a ubiquitination-phosphoprotein axis in chromatin during neurodevelopment.
Authors: Authors: Ledvin L, Gassaway BM, Tawil J, Urso O, Pizzo D, Welsh KA, Bolhuis DL, Fisher D, Bonni A, Gygi SP, Brown NG, Ferguson CJ.
Dev Cell
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