Headshot of Ed Chouchani

Edward Chouchani, Ph.D.

Professor of Cancer Biology (Dana-Farber Cancer Institute)
Professor of Cell Biology (HMS)
HHMI Investigator

Edward Chouchani, Ph.D., joined the faculty of Harvard Medical School in 2017. He received his Ph.D. in Biological Sciences at the University of Cambridge and MRC Mitochondrial Biology Unit. He then performed postdoctoral research at the Dana-Farber Cancer Institute and Harvard Medical School. In 2024, he became a Howard Hughes Medical Institute Investigator.

Research in the Chouchani Lab focuses on deciphering molecular mechanisms that drive metabolic disease, and using this information to develop targeted therapeutic strategies.  Mitochondria are critical hubs for metabolic signaling, and their dysfunction is key in the pathology of metabolic disease.  The Chouchani Lab combines mass spectrometry and targeted pharmacological approaches in vivo to understand how mitochondrial redox metabolism controls physiology in clinically informative mouse models of obesity and diabetes.

Dana Farber Cancer Institute

Dept. of Cell Biology, LC-6212

360 Longwood Avenue

Boston, MA 02115

Lab Phone: 617-632-3281

Lab Fax: 617-632-5363

Multiplexed Isobaric Tag-Based Profiling of Seven Murine Tissues Following In Vivo Nicotine Treatment Using a Minimalistic Proteomics Strategy.
Authors: Authors: Paulo JA, Jedrychowski MP, Chouchani ET, Kazak L, Gygi SP.
Proteomics
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Itaconate is an anti-inflammatory metabolite that activates Nrf2 via alkylation of KEAP1.
Authors: Authors: Mills EL, Ryan DG, Prag HA, Dikovskaya D, Menon D, Zaslona Z, Jedrychowski MP, Costa ASH, Higgins M, Hams E, Szpyt J, Runtsch MC, King MS, McGouran JF, Fischer R, Kessler BM, McGettrick AF, Hughes MM, Carroll RG, Booty LM, Knatko EV, Meakin PJ, Ashford MLJ, Modis LK, Brunori G, Sévin DC, Fallon PG, Caldwell ST, Kunji ERS, Chouchani ET, Frezza C, Dinkova-Kostova AT, Hartley RC, Murphy MP, O'Neill LA.
Nature
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Mitochondrial reactive oxygen species and adipose tissue thermogenesis: Bridging physiology and mechanisms.
Authors: Authors: Chouchani ET, Kazak L, Spiegelman BM.
J Biol Chem
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Genetic Depletion of Adipocyte Creatine Metabolism Inhibits Diet-Induced Thermogenesis and Drives Obesity.
Authors: Authors: Kazak L, Chouchani ET, Lu GZ, Jedrychowski MP, Bare CJ, Mina AI, Kumari M, Zhang S, Vuckovic I, Laznik-Bogoslavski D, Dzeja P, Banks AS, Rosen ED, Spiegelman BM.
Cell Metab
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Genetic Depletion of Adipocyte Creatine Metabolism Inhibits Diet-Induced Thermogenesis and Drives Obesity.
Authors: Authors: Kazak L, Chouchani ET, Lu GZ, Jedrychowski MP, Bare CJ, Mina AI, Kumari M, Zhang S, Vuckovic I, Laznik-Bogoslavski D, Dzeja P, Banks AS, Rosen ED, Spiegelman BM.
Cell Metab
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Identification and quantification of protein S-nitrosation by nitrite in the mouse heart during ischemia.
Authors: Authors: Chouchani ET, James AM, Methner C, Pell VR, Prime TA, Erickson BK, Forkink M, Lau GY, Bright TP, Menger KE, Fearnley IM, Krieg T, Murphy MP.
J Biol Chem
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UCP1 deficiency causes brown fat respiratory chain depletion and sensitizes mitochondria to calcium overload-induced dysfunction.
Authors: Authors: Kazak L, Chouchani ET, Stavrovskaya IG, Lu GZ, Jedrychowski MP, Egan DF, Kumari M, Kong X, Erickson BK, Szpyt J, Rosen ED, Murphy MP, Kristal BS, Gygi SP, Spiegelman BM.
Proc Natl Acad Sci U S A
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Mitochondrial Patch Clamp of Beige Adipocytes Reveals UCP1-Positive and UCP1-Negative Cells Both Exhibiting Futile Creatine Cycling.
Authors: Authors: Bertholet AM, Kazak L, Chouchani ET, Bogaczynska MG, Paranjpe I, Wainwright GL, Bétourné A, Kajimura S, Spiegelman BM, Kirichok Y.
Cell Metab
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Stress turns on the heat: Regulation of mitochondrial biogenesis and UCP1 by ROS in adipocytes.
Authors: Authors: Ortega SP, Chouchani ET, Boudina S.
Adipocyte
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Corrigendum: Mitochondrial ROS regulate thermogenic energy expenditure and sulfenylation of UCP1.
Authors: Authors: Chouchani ET, Kazak L, Jedrychowski MP, Lu GZ, Erickson BK, Szpyt J, Pierce KA, Laznik-Bogoslavski D, Vetrivelan R, Clish CB, Robinson AJ, Gygi SP, Spiegelman BM.
Nature
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