Wade Harper, Ph.D.

Wade Harper, Ph.D.

Bert and Natalie Vallee Professor of Molecular Pathology (HMS)
Professor of Cell Biology (HMS)
Chair of the Department of Cell Biology (HMS)

Wade Harper, Ph.D., is the B and N Vallee Professor of Molecular Pathology, a Professor of Cell Biology, and the Chair of Cell Biology in the Blavatnik Institute at Harvard Medical School. He received his Ph.D. in Chemistry from Georgia Institute of Technology, prior to performing post-doctoral work in protein biochemistry of growth factors at Harvard Medical School. He joined the faculty in the Department of Biochemistry and Molecular Biology at Baylor College of Medicine in 1988 and subsequently moved to the Department of Pathology at Harvard Medical School (in 2003) and to the Department of Cell Biology in 2011.

The Harper Lab studies mechanisms underlying cellular homeostasis and signaling, with a focus on the ubiquitin system and the autophagy-lysosome system. The interest in the ubiquitin-proteasome system in the Harper Lab initially emerged through studies to understand how cell cycle regulators (cyclins and CDK inhibitors) are degraded to control cell cycle transitions, resulting in the discovery of cullin-RING ubiquitin ligases, and their roles in phosphorylation-dependent protein degradation. The Harper Lab currently uses quantitative proteomics, imaging, and biochemical approaches to elucidate underlying biochemical mechanisms controlling protein turnover, and applies these approaches to examine regulatory pathways relevant to various neurodegenerative disease, including Parkinson’s and Alzheimer’s diseases. A major focus currently is the PARKIN ubiquitin ligase, which controls turnover of damaged mitochondria via the autophagy pathway and is mutated in Parkinson’s Disease. The Harper Lab, together with the Gygi Lab at HMS, is also using proteomics to develop a large-scale human protein interaction network including the majority of proteins encoded by the human genome.

Harvard Medical School

Dept. of Cell Biology, C-462A

240 Longwood Avenue

Boston, MA 02115

Lab telephone: 617-432-6590

Lab fax: 617-432-6591

Ribosome Abundance Control Via the Ubiquitin-Proteasome System and Autophagy.
Authors: Authors: An H, Harper JW.
J Mol Biol
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The role of nuclear receptor co-activator 4 in erythropoiesis (Reply to Nai et al.).
Authors: Authors: Santana-Codina N, Gableske S, <a href="https://connects.catalyst.harvard.edu/Profiles/profile/1245794">Fleming MD</a>, Harper JW, Kimmelman AC, <a href="https://connects.catalyst.harvard.edu/Profiles/profile/1244106">Mancias JD</a>.
Haematologica
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Acute unfolding of a single protein immediately stimulates recruitment of ubiquitin protein ligase E3C (UBE3C) to 26S proteasomes.
Authors: Authors: Gottlieb CD, Thompson ACS, Ordureau A, Harper JW, Kopito RR.
J Biol Chem
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Integrated proteogenetic analysis reveals the landscape of a mitochondrial-autophagosome synapse during PARK2-dependent mitophagy.
Authors: Authors: Heo JM, Harper NJ, <a href="https://connects.catalyst.harvard.edu/Profiles/profile/1231877">Paulo JA</a>, Li M, Xu Q, Coughlin M, <a href="https://connects.catalyst.harvard.edu/Profiles/profile/1240445">Elledge SJ</a>, Harper JW.
Sci Adv
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Mitochondrial Reprogramming Underlies Resistance to BCL-2 Inhibition in Lymphoid Malignancies.
Authors: Authors: Guièze R, Liu VM, Rosebrock D, Jourdain AA, Hernández-Sánchez M, Martinez Zurita A, Sun J, <a href="https://connects.catalyst.harvard.edu/Profiles/profile/67352113">Ten Hacken E</a>, Baranowski K, Thompson PA, Heo JM, Cartun Z, Aygün O, <a href="https://connects.catalyst.harvard.edu/Profiles/profile/59364113">Iorgulescu JB</a>, Zhang W, <a href="https://connects.catalyst.harvard.edu/Profiles/profile/105867204">Notarangelo G</a>, Livitz D, Li S, <a href="https://connects.catalyst.harvard.edu/Profiles/profile/1232164">Davids MS</a>, <a href="https://connects.catalyst.harvard.edu/Profiles/profile/84891873">Biran A</a>, Fernandes SM, <a href="https://connects.catalyst.harvard.edu/Profiles/profile/1241474">Brown JR</a>, Lako A, Ciantra ZB, Lawlor MA, Keskin DB, Udeshi ND, Wierda WG, Livak KJ, <a href="https://connects.catalyst.harvard.edu/Profiles/profile/1246239">Letai AG</a>, <a href="https://connects.catalyst.harvard.edu/Profiles/profile/1241052">Neuberg D</a>, Harper JW, Carr SA, Piccioni F, <a href="https://connects.catalyst.harvard.edu/Profiles/profile/1240689">Ott CJ</a>, Leshchiner I, Johannessen CM, Doench J, <a href="https://connects.catalyst.harvard.edu/Profiles/profile/1229689">Mootha VK</a>, <a href="https://connects.catalyst.harvard.edu/Profiles/profile/26613063">Getz G</a>, <a href="https://connects.catalyst.harvard.edu/Profiles/profile/1249628">Wu CJ</a>.
Cancer Cell
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Hook3 is a scaffold for the opposite-polarity microtubule-based motors cytoplasmic dynein-1 and KIF1C.
Authors: Authors: Kendrick AA, Dickey AM, Redwine WB, Tran PT, Vaites LP, Dzieciatkowska M, Harper JW, Reck-Peterson SL.
J Cell Biol
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The PINK1 kinase-driven ubiquitin ligase Parkin promotes mitochondrial protein import through the presequence pathway in living cells.
Authors: Authors: Jacoupy M, Hamon-Keromen E, Ordureau A, Erpapazoglou Z, Coge F, Corvol JC, Nosjean O, Mannoury la Cour C, Millan MJ, Boutin JA, Harper JW, Brice A, Guedin D, Gautier CA, Corti O.
Sci Rep
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Protein aggregation mediates stoichiometry of protein complexes in aneuploid cells.
Authors: Authors: Brennan CM, Vaites LP, Wells JN, Santaguida S, <a href="https://connects.catalyst.harvard.edu/Profiles/profile/1231877">Paulo JA</a>, Storchova Z, Harper JW, Marsh JA, Amon A.
Genes Dev
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ARIH2 Is a Vif-Dependent Regulator of CUL5-Mediated APOBEC3G Degradation in HIV Infection.
Authors: Authors: Hüttenhain R, Xu J, Burton LA, Gordon DE, Hultquist JF, Johnson JR, Satkamp L, Hiatt J, Rhee DY, Baek K, Crosby DC, Frankel AD, Marson A, Harper JW, Alpi AF, Schulman BA, Gross JD, Krogan NJ.
Cell Host Microbe
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A glycine-specific N-degron pathway mediates the quality control of protein N-myristoylation.
Authors: Authors: Timms RT, Zhang Z, Rhee DY, Harper JW, Koren I, <a href="https://connects.catalyst.harvard.edu/Profiles/profile/1240445">Elledge SJ</a>.
Science
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