Radhika Subramanian

Radhika Subramanian, Ph.D.

Assistant Professor of Genetics, Massachusetts General Hospital
Assistant Molecular Biologist (MGH)
Affiliate Member of Cell Biology

Radhika received her Ph.D. in Biochemistry from Brandeis University. She then performed postdoctoral work at the Rockefeller University. She joined the faculty of the Massachusetts General Hospital and Harvard Medical School in October 2014.

The Subramanian Lab is interested in how micron-length scale structures that are critical for cellular signaling emerge from the collective activity of nanometer-sized proteins. We address this question in the context of microtubule organization for (1) cell division and (2) cilium-dependent Hedgehog signal transduction. We primarily use a reconstitution-based approach and ‘reconstruct’ sub-reactions of these cellular pathways in vitro from purified components. We aim to decipher the fundamental rules that govern the spatial-temporal organization of cellular machines and organelles, such as the spindle and the cilium, through this approach. We employ a wide range of experimental techniques, integrating information from cutting-edge single-molecule methods, high-resolution microscopy, structural tools, and biochemical and cellular read-outs. Through these studies, our goal is to understand the cellular mechanisms relevant to developmental disorders and human cancers.

Simches Research Center

Massachusetts General Hospital

185 Cambridge St. CPZN-7600

Boston, MA 02114

Collaborative role of two distinct cilium-specific cytoskeletal systems in driving Hedgehog-responsive transcription factor trafficking.
Authors: Authors: Ku PI, Sreeja JS, Chadha A, Williams DS, Engelke MF, Subramanian R.
Sci Adv
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Non-canonical CDK6 activity promotes cilia disassembly by suppressing axoneme polyglutamylation.
Authors: Authors: He K, Sun X, Chen C, Luc S, Robichaud JH, Zhang Y, Huang Y, Ji B, Ku PI, Subramanian R, Ling K, Hu J.
J Cell Biol
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Collaborative role of two distinct cilium-specific cytoskeletal systems in driving Hedgehog-responsive transcription factor trafficking.
Authors: Authors: Ku PI, Sreeja JS, Chadha A, Williams DS, Engelke MF, Subramanian R.
bioRxiv
View full abstract on Pubmed
Architecture of native kinetochores revealed by structural studies utilizing a thermophilic yeast.
Authors: Authors: Barrero DJ, Wijeratne SS, Zhao X, Cunningham GF, Yan R, Nelson CR, Arimura Y, Funabiki H, Asbury CL, Yu Z, Subramanian R, Biggins S.
Curr Biol
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Torques within and outside the human spindle balance twist at anaphase.
Authors: Authors: Neahring L, Cho NH, He Y, Liu G, Fernandes J, Rux CJ, Nakos K, Subramanian R, Upadhyayula S, Yildiz A, Dumont S.
J Cell Biol
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GRK2 kinases in the primary cilium initiate SMOOTHENED-PKA signaling in the Hedgehog cascade.
Authors: Authors: Walker MF, Zhang J, Steiner W, Ku PI, Zhu JF, Michaelson Z, Yen YC, Lee A, Long AB, Casey MJ, Poddar A, Nelson IB, Arveseth CD, Nagel F, Clough R, LaPotin S, Kwan KM, Schulz S, Stewart RA, Tesmer JJG, Caspary T, Subramanian R, Ge X, Myers BR.
PLoS Biol
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Modularity of PRC1 composition and chromatin interaction define condensate properties.
Authors: Authors: Niekamp S, Marr SK, Oei TA, Subramanian R, Kingston RE.
Mol Cell
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Architecture and flexibility of native kinetochores revealed by structural studies utilizing a thermophilic yeast.
Authors: Authors: Barrero DJ, Wijeratne SS, Zhao X, Cunningham GF, Rui Y, Nelson CR, Yasuhiro A, Funabiki H, Asbury CL, Yu Z, Subramanian R, Biggins S.
bioRxiv
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Cytoskeleton crosstalk: Casting stable actin bundles with dynamic microtubule molds.
Authors: Authors: Haque F, Subramanian R.
Curr Biol
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Modularity of PRC1 Composition and Chromatin Interaction define Condensate Properties.
Authors: Authors: Niekamp S, Marr SK, Oei TA, Subramanian R, Kingston RE.
bioRxiv
View full abstract on Pubmed