Headshot of Ed Chouchani

Edward Chouchani, Ph.D.

Professor of Cancer Biology (Dana-Farber Cancer Institute)
Professor of Cell Biology (HMS)
HHMI Investigator

Edward Chouchani, Ph.D., joined the faculty of Harvard Medical School in 2017. He received his Ph.D. in Biological Sciences at the University of Cambridge and MRC Mitochondrial Biology Unit. He then performed postdoctoral research at the Dana-Farber Cancer Institute and Harvard Medical School. In 2024, he became a Howard Hughes Medical Institute Investigator.

Research in the Chouchani Lab focuses on deciphering molecular mechanisms that drive metabolic disease, and using this information to develop targeted therapeutic strategies.  Mitochondria are critical hubs for metabolic signaling, and their dysfunction is key in the pathology of metabolic disease.  The Chouchani Lab combines mass spectrometry and targeted pharmacological approaches in vivo to understand how mitochondrial redox metabolism controls physiology in clinically informative mouse models of obesity and diabetes.

Dana Farber Cancer Institute

Dept. of Cell Biology, LC-6212

360 Longwood Avenue

Boston, MA 02115

Lab Phone: 617-632-3281

Lab Fax: 617-632-5363

Mitochondria selective S-nitrosation by mitochondria-targeted S-nitrosothiol protects against post-infarct heart failure in mouse hearts.
Authors: Authors: Methner C, Chouchani ET, Buonincontri G, Pell VR, Sawiak SJ, Murphy MP, Krieg T.
Eur J Heart Fail
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Using exomarkers to assess mitochondrial reactive species in vivo.
Authors: Authors: Logan A, Cochemé HM, Li Pun PB, Apostolova N, Smith RA, Larsen L, Larsen DS, James AM, Fearnley IM, Rogatti S, Prime TA, Finichiu PG, Dare A, Chouchani ET, Pell VR, Methner C, Quin C, McQuaker SJ, Krieg T, Hartley RC, Murphy MP.
Biochim Biophys Acta
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Complex I deficiency due to selective loss of Ndufs4 in the mouse heart results in severe hypertrophic cardiomyopathy.
Authors: Authors: Chouchani ET, Methner C, Buonincontri G, Hu CH, Logan A, Sawiak SJ, Murphy MP, Krieg T.
PLoS One
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Cardioprotection by S-nitrosation of a cysteine switch on mitochondrial complex I.
Authors: Authors: Chouchani ET, Methner C, Nadtochiy SM, Logan A, Pell VR, Ding S, James AM, Cochemé HM, Reinhold J, Lilley KS, Partridge L, Fearnley IM, Robinson AJ, Hartley RC, Smith RA, Krieg T, Brookes PS, Murphy MP.
Nat Med
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Inactivation of pyruvate dehydrogenase kinase 2 by mitochondrial reactive oxygen species.
Authors: Authors: Hurd TR, Collins Y, Abakumova I, Chouchani ET, Baranowski B, Fearnley IM, Prime TA, Murphy MP, James AM.
J Biol Chem
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Mitochondrial redox signalling at a glance.
Authors: Authors: Collins Y, Chouchani ET, James AM, Menger KE, Cochemé HM, Murphy MP.
J Cell Sci
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Proteomic approaches to the characterization of protein thiol modification.
Authors: Authors: Chouchani ET, James AM, Fearnley IM, Lilley KS, Murphy MP.
Curr Opin Chem Biol
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Quantification and identification of mitochondrial proteins containing vicinal dithiols.
Authors: Authors: Requejo R, Chouchani ET, James AM, Prime TA, Lilley KS, Fearnley IM, Murphy MP.
Arch Biochem Biophys
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Identification of S-nitrosated mitochondrial proteins by S-nitrosothiol difference in gel electrophoresis (SNO-DIGE): implications for the regulation of mitochondrial function by reversible S-nitrosation.
Authors: Authors: Chouchani ET, Hurd TR, Nadtochiy SM, Brookes PS, Fearnley IM, Lilley KS, Smith RA, Murphy MP.
Biochem J
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Measuring mitochondrial protein thiol redox state.
Authors: Authors: Requejo R, Chouchani ET, Hurd TR, Menger KE, Hampton MB, Murphy MP.
Methods Enzymol
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